Journal
JOURNAL OF MICROBIOLOGY
Volume 57, Issue 2, Pages 127-137Publisher
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-019-8398-y
Keywords
white rot fungi; laccase; mycelial interactions; transcriptome analysis; Dichomitus squalens
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Funding
- National Natural Foundation of China [31400063]
- Fundamental Research Funds for the Central Universities [XDJK2011B009, XDJK2017B030]
- Research Funds of Scientific Platform and Base Construction [cstc2014pt-sy0017]
- Chongqing Research Program of Social undertaking and livelihood security [cstc2016shmszx1176]
- Recruitment Program for Foreign Experts [WQ20125500073]
- State Bureau of Forestry 948 project [2015-4-42]
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Interspecific mycelial interactions between white rot fungi are always accompanied by an increased production of laccase. In this study, the potential of the white rot fungus Dichomitus squalens to enhance laccase production during interactions with two other white rot fungi, Trametes versicolor or Pleurotus ostreatus, was assessed. To probe the mechanism of laccase induction and the role that laccase plays during combative interaction, we analyzed the differential gene expression profile of the laccase induction response to stressful conditions during fungal interaction. We further confirmed the expression patterns of 16 selected genes by qRT-PCR analysis. We noted that many differentially expressed genes (DEGs) encoded proteins that were involved in xenobiotic detoxification and reactive oxygen species (ROS) generation or reduction, including aldo/keto reductase, glutathione S-transferases, cytochrome P450 enzymes, alcohol oxidases and dehydrogenase, manganese peroxidase and laccase. Furthermore, many DEG-encoded proteins were involved in antagonistic mechanisms of nutrient acquisition and antifungal properties, including glycoside hydrolase, glucanase, chitinase and terpenoid synthases. DEG analyses effectively revealed that laccase induction was likely caused by protective responses to oxidative stress and nutrient competition during interspecific fungal interactions.
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