Journal
ANALYST
Volume 144, Issue 2, Pages 463-467Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8an01486h
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Funding
- National Natural Science Foundation of China [21702053, 21676075, 21702070]
- Hubei Provincial Department of Education project [Q20171013]
- China Postdoctoral Science Foundation [2017M612461]
- Hubei Provincial Natural Science Foundation of China [2016CFB126]
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Leucine aminopeptidase (LAP), one of the important cancer-related biomarkers, is significantly overexpressed in many malignant tumor cells. Developing an effective fluorescent probe for high-specificity and in situ trapping of endogenous LAP in living samples is still challenging. In this project, we report a water-soluble near-infrared (NIR) fluorescent probe (CHMC-M-Leu) for specific monitoring of LAP in vitro and in vivo. The novel fluorescent probe (CHMC-M-Leu) contains a NIR-emitting fluorophore (CHMC-M) as the reporter and L-leucine as the enzyme-active trigger moiety which are linked together by a p-aminobenzyl alcohol (PABA) section. Upon exposure to LAP, the fluorescence at 625 nm gets impressively enhanced, which belongs to the near-infrared region and is beneficial for imaging in vivo. Furthermore, the novel fluorescent probe exhibits fast response and highly chemoselective detection of LAP in various bio-related species. In addition, CHMC-M-Leu shows favourable cellular uptake and was successfully used to monitor endogenous LAP in living cells.
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