Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 59, Issue -, Pages 56-59Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2015.07.009
Keywords
Electrochemiluminescence; Resonance energy transfer; CdS:Eu NCs; Ru(bpy)(3)(2+)-doped silica nanoparticle; Prostate-specific antigen
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Funding
- 973 Program [2012CB932600]
- National Natural Science Foundation of China [21135003, 21475058]
- Science and Technology Support Project [BE2013073]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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A novel electrochemiluminescence resonance energy transfer (ECL-RET) system from Eu3+-doped CdS nanocrystals (CdS:Eu NCs) to Ru(bpy)(3)(2+)-doped silica nanoparticle (RuSi) was designed for ultrasensitive detection of prostate-specific antigen (PSA). The platform consists of a CdS:Eu NCs film on glassy carbon electrode (GCE) as ECL emitter and a sandwich-type immunocomplex formed by the primary antibody, PSA and RuSi-tagged second antibody. This system showed a maximum of 4.83-fold enhancement of ECL intensity at 620 nm due to the efficient ECL-RET and high Ru(bpy)(3)(2+) quantum yields. By the signal amplification of RuSi and the specific antibody-antigen interactions, this ECL-RET system could sensitively respond down to 1.0 fg mL(-1) PSA. (C) 2015 Elsevier B.V. All rights reserved.
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