An ICT-Based Mitochondria-Targeted Fluorescent Probe for Hydrogen Peroxide with a Large Turn-On Fluorescence Signal

An ICT-based mitochondria-targeted fluorescent probe Mito-NP was designed and synthesized for the detection of H2O2, with radical fluorescence intensity changes (up to 120-fold) and a large Stokes shift (93 nm) in aqueous solution. There was good linear relationship (R-2=0.9983) between the concentration of H2O2 in the range of 0 to 100 mu M and the fluorescence intensity at 553 nm, with a low detection limit of 0.25 mu M (S/N=3). Mito-NP could also visualize enzymatically generated H2O2. Furthermore, the probe could detect endogenous H2O2 with good mitochondria targeting ability in living cells and could also detect the change in H2O2 levels during mitochondria-oriented apoptosis. Introduction