Journal
MICROSCOPY RESEARCH AND TECHNIQUE
Volume 80, Issue 11, Pages 1221-1228Publisher
WILEY
DOI: 10.1002/jemt.22920
Keywords
phase-contrast X-ray; skeletal muscle; soleus; synchrotron
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Funding
- Soonchunhyang University Research Fund
- Basic Science Research Program through the National Research Foundation of Korea (NRF)
- Ministry of Science, ICT, and Future Planning, Republic of Korea [2016R1C1B1016492]
- National Research Foundation of Korea [2016R1C1B1016492] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Radiation beam interface contrast X-ray microscopy provides resolution of a few dozen nanometers from fixed whole muscle biopsies, allowing better reconstruction of the microstructure of the muscle than is currently possible with classic histological techniques. Fixed soleus muscle biopsies have been evaluated from the walk-in mouse model using phase-contrast X-ray microscopy, and results presented that corroborate the accuracy of the method used, and its potential for application in physiotherapy and occupational therapy studies. We believe that this method will enhance existing morphometric methods of analysis, leading to accurate reconstruction of other thick specimens that would otherwise require thin sectioning and reconstruction through deconvolution algorithms.
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