Journal
ELECTROANALYSIS
Volume 27, Issue 9, Pages 2131-2138Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201500098
Keywords
Graphene; Biosensor; Dendrimer; Hybrid nanomaterial; Tyrosinase
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Funding
- Spanish Ministerio de Ciencia e Innovacion [CTQ2011-24355, CTQ2012-34238]
- Comunidad de Madrid, Programme NANOAVANSENS [S2013/MIT-3029]
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A novel nanostructured architecture for the construction of electrochemical enzyme biosensors is here described. It implies the electrostatic layer-by-layer assembly of four-generation ethylenediamine core polyamidoamine G-4 dendrimers on glassy carbon electrodes coated with a graphene oxide-carboxymethylcellulose hybrid nanomaterial. This modified surface was further employed for the covalent immobilization of the model enzyme tyrosinase through a glutaraldehyde-mediated cross-linking. The prepared enzyme electrode allowed the amperometric detection of catechol in the 2-400 nM range. The biosensor showed excellent analytical performance with high sensitivity of 6.3 A/M and low detection limit of 0.9 nM. The enzyme electrode retained over 93% of the initial activity after 40 days at 4 degrees C.
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