Journal
ELECTROANALYSIS
Volume 28, Issue 1, Pages 161-168Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201500501
Keywords
Photoelectrochemical Aptasensor; Graphene Functionalized TiO2 Nanotubes; Microcystin-LR
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Funding
- National Natural Science Foundation of China (NSFC) [21377092, 21277099, 21477085]
- Science & Technology Commission of Shanghai Municipality [14DZ2261100]
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In this work, a visible-light driven photoelectrochemical (PEC) aptasensor with high sensitivity and selectivity for cyanobacterial toxin Microcystin-LR (MC-LR) has been developed. MC-LR aptamers, serving as the specific recognition element, were conveniently immobilized onto vertically-aligned titanium dioxide nanotubes (TiO2 NTs) photoanode substrate with the aid of graphene via - stacking interactions between the graphene hexagonal cells and the nucleobases of the DNA. The functionalization of graphene further endowed the constructed aptasensor visible-light response activity. The aptasensor was used for the label-free analysis of MC-LR by monitoring the increase in photocurrent that resulted from the binding of MC-LR and its aptamers. Results showed that the photocurrent increment had a linear relationship with logarithm of MC-LR concentration in the range from 1.0 to 500fM and the limit of detection reached as low as 0.5fM. Furthermore, the aptasensor exhibited high selectivity while used to detect 100-fold concentration other several potential coexisting interferents. A promising PEC aptasensing platform for highly sensitive and selective monitoring of MC-LR has thus been established.
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