Journal
PLANT SIGNALING & BEHAVIOR
Volume 14, Issue 2, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15592324.2018.1561107
Keywords
Arabidopsis thaliana; red light; photosynthesis; guard cells; immunohistochemistry; phosphorylation; plasma membrane H+-ATPase
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [15H05956, 15H04386]
- [14J00303]
- Grants-in-Aid for Scientific Research [15H05956, 15H04386] Funding Source: KAKEN
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Stomatal opening is induced by red light as well as blue light. Recently, we established an immunohistochemical technique using whole leaves to study plasma membrane (PM) H+-ATPase in guard cells, which is an important enzyme driving stomatal opening. Our technique revealed that red light illuminated to whole leaves induces photosynthesis-dependent phosphorylation of C-terminal penultimate residue of PM H+-ATPase, threonine, in guard cells, which has been considered to be important for activation of PM H+-ATPase, and we proposed that red light promotes stomatal opening via activation of PM H+-ATPase in guard cells in whole leaves. Here, using our new immunohistochemical technique, we investigated fluence rate dependence of red light-induced phosphorylation of PM H+-ATPase. We found that illumination of red light at 50 mu mol m(-2)s(-1), which was suggested to initiate photosynthesis, saturates phosphorylation of PM H+-ATPase. Furthermore, we immunohistochemically confirmed decrease in the amount of PM H+-ATPase protein in a knock-out mutant of AHA1, an isogene encoding the major isoform of PM H+-ATPase in guard cells, implying the importance of AHA1 as the major PM H+-ATPase protein in guard cells for light-induced stomatal opening.
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