Journal
CHEMICAL COMMUNICATIONS
Volume 55, Issue 5, Pages 707-710Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8cc08796b
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Funding
- UMass Amherst
- NIH [T32GM008515, R01AI136789]
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Silver has been widely used for disinfection. The cellular accumulation of silver ions (Ag+) is critical in these antibacterial effects. The direct cellular measurement of Ag+ is useful for the study of disinfection mechanisms. Herein, we reported a novel genetically encoded RNA-based sensor to image Ag+ in live bacterial cells. The sensor is designed by introducing a cytosine-Ag+-cytosine metallo base pair into a fluorogenic RNA aptamer, Broccoli. The binding of Ag+ induces the folding of Broccoli and activates a fluorescence signal. This sensor can be genetically encoded to measure the cellular flux and antibacterial effect of Ag+.
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