Journal
BIO-PROTOCOL
Volume 9, Issue 2, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.3146
Keywords
Insulin; Adipose tissue; Muscle; Glucose transport; Glucose uptake; Insulin tolerance test
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Funding
- National Health and Medical Research Council of Australia (NHMRC) [1061122, 1086850]
- National Health and Medical Research Council of Australia Senior Principal Research Fellow
- National Health and Medical Research Council of Australia [1061122, 1086850] Funding Source: NHMRC
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Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.
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