Journal
ELECTROANALYSIS
Volume 27, Issue 11, Pages 2679-2687Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201500169
Keywords
Fumonisin B1; Electrochemical immunosensor; Sensitive detection; Single-walled carbon nanotubes/chitosan; Corn
Categories
Funding
- National Natural Science Foundation of China [31071093, 31170129, 31200064]
- Science and Technology Planning Project of YuZhong District of Chongqing City, China [20140119]
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A sensitive electrochemical immunosensor was developed for detecting fumonisin B-1 (FB1) in corn using the single-walled carbon nanotubes/chitosan. The detection mechanism of immunosensor was based on an indirect competitive binding to a fixed amount of anti-FB1 between free FB1 and FB1-bovine serum albumin, which was conjugated on covalently functionalized nanotubes/chitosan laid on the glass carbon electrode. The anti-rabbit immunoglobulin G secondary antibody labeled with alkaline phosphatase was then bound to the electrode surface through reactisubstrate alpha-naphthyl phosphate, which produced electrochemical signal. Under optimized conditions, this method could detect FB1 from 0.01 to 1000 ngmL(-1) with a detection limit of 2 pgmL(-1). This is well below the detection limit required from European Union legislation, 2-4 mgL(-1). Moreover, good recoveries were obtained for the detection of spiked corn samples and actual corn samples. As the method has good sensitivity and recovery for detecting FB1, it is a practical detection method.
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