Journal
MICROPOROUS AND MESOPOROUS MATERIALS
Volume 239, Issue -, Pages 158-166Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micromeso.2016.09.006
Keywords
Mesoporous TiO2; Adenosine deaminase; Microparticles; Amino; Immobilization
Categories
Funding
- National Outstanding Youth Foundation of China [21025625]
- National High Tech Research and Development Program of China (863) [2012AA021200]
- National Basic Research Program of China (973) [2011CBA00806, 2013CB733503]
- National Key Technology RD Program [2012BAI44G01]
- National Natural Science Foundation of China [21506090, 201390204]
- Natural Science Foundation of Jiangsu Grants [BK20130929, BK20140940]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Fabrication of biocompatible micro- and nanoparticles is attractive because of their potential for application as enzyme immobilization tools. Mesoporous TiO2 microparticles with high crystallinity, high hydroxyl density, and large pore size (20 nm) were prepared by solid-state calcination and a soft chemistry method. The large pores of the microparticles were efficient in adenosine deaminase (ADA) encapsulation. The hydroxyl-coated microparticles could optimize amino-silane modification and be efficiently utilized as ADA-immobilization carriers. However, the adsorbed enzymes were easily leached when cycled. Sequential application of the coupling agent 3-aminopropyltriethoxysilane and cross-linker glutaraldehyde (GLU) enabled effective ADA coupling. After eight batch cycles, the immobilized ADA retained 80% of its initial activity, much higher than that by direct enzyme adsorption (30%). GLU prevented enzyme desorption and loss of activity. We thus improved ADA loading efficiency, recycling, and stability. TiO2 microparticles could be suitable ADA immobilization candidates for detection and industrial inosinic acid production. (C) 2016 Elsevier Inc. All rights reserved.
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