Journal
MICROCHIMICA ACTA
Volume 185, Issue 1, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-017-2532-5
Keywords
Screen-printed electrode; Copper sulfide; Carbohydrate antigen 15-3; Tumor biomarkers; Immunosensors; Electrochemistry; Biosensor
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Funding
- National Institute for Medical Research Development (NIMAD) [957254]
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The authors report on an electrochemical immunosensor for the tumor marker carbohydrate antigen 15-3 (CA15-3). It is based on the use of a composite consisting of reduced graphene oxide (RGO) and copper sulfide (CuS) that was placed on a screen-printed graphite electrode. The electrode shows excellent activity towards the oxidation of catechol acting as an electrochemical probe, best at a working potential of 0.16 V. The electrode was modified with antibody against CA15-3. Once the analyte (CA15-3) binds to the surface of the electrode, the response to catechol is reduced. The assay has a linear response in the 1.0-150 U mL(-1) CA15-3 concentration range, with a 0.3 U mL(-1) lower detection limit and a sensitivity of 1.88 mu A mu M-1 cm(-2). The immunosensor also shows good reproducibility (2.7%), stability (95% of the initial values after storing for four weeks). The method was successfully applied to the determination of CA15-3 in serum samples, and results were found to compare well to those obtained by an ELISA. Conceivably, this nanocomposite based detection scheme has a wider scope and may be applied to numerous other immunoassays.
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