Journal
MICROCHIMICA ACTA
Volume 184, Issue 7, Pages 2357-2363Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-017-2209-0
Keywords
Nanocomposite; Semiconductor nanomaterials; Noble metal nanoparticles; Modified electrode; Glucose oxidase; Direct electron transfer
Categories
Funding
- National Natural Science Foundation of China [21475116, 21575125, 21575124, 81302016]
- Qinglan Project of Jiangsu Province
- high-end talent supporting plan of Yangzhou University
- Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD)
- National Natural Science Foundation of Jiangsu Province [BK20130455, BK20140101]
- Six Talent Peaks Project in Jiangsu Province [WSN-106]
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The authors have prepared a platinum nanoparticle-assembled nanoflake-like SnS2 nanocomposite of the type PtNP@SnS2 as a support for immobilization of glucose oxidase (GOx) to obtain an electrochemical glucose biosensor based on direct electron transfer of GOx. Scanning electron microscopy, transmission electron microscopy, X-ray diffraction, electrochemical impedance spectroscopy, static water contact angle measurements, and cyclic voltammetry were used to characterize the nanocomposite and the glucose biosensor. The nanocomposite has a large surface-to-volume ratio, excellent hydrophiliciy, and high electrochemical activity. This promotes the direct electron transfer between GOx and the surface of a glassy carbon electrode (GCE). The GOx immobilized on the nanocomposite displays good bioactivity, and the surface coverage of the modified electrode is 4.15 x 10(-11) mol center dot cm(-2). Under optimum conditions and at a working voltage of -0.4 V vs. SCE, the glucose biosensor exhibits a linear response in the 0.1 to 1.0 mM and 1.0 to 12 mM glucose concentration ranges, with a lower detection limit of 2.5 mu M (at an S/N ratio of 3). The biosensor shows excellent selectivity and was successfully applied to the determination of glucose in human serum samples.
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