Journal
MICROCHEMICAL JOURNAL
Volume 132, Issue -, Pages 28-35Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2016.12.021
Keywords
Passiflora; Flavonoids; Accelerated solvent extraction; Box-Behnken design; Multi-response optimization
Categories
Funding
- CNPq (Conselho Nacional do Desenvolvimento Cientifico e Tecnologico) [472605/2012-7]
- FAPESB (Fundacao de Apoio a Pesquisa do Estado da Bahia) [PNE0008/2011]
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This work describes the development of a method for the extraction of phenolic compounds, mainly fiavonoids, from species of Passiflora by employing accelerated solvent extraction (ASE) and using a Box-Behnken design with desirability functions for optimization. The optimal extraction conditions consisted of an extraction temperature of 80 degrees C, 64% (w/w) ethanol and five number of extraction cycles. A high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of orientin, isoorientin, vitexin, isovitexin and rutin flavonoids in the leaves of seventeen Passiflora spp. using gradient elution with acetonitrile (solvent B) and 0.2% (w/w) formic acid in water (solvent A) as the eluent mixture was also developed and validated. These five fiavonoids were quantified with good linearity, LOQs, LODs, precision, and accuracy. Higher concentrations of isoorientin, orientin, vitexin, isovitexin and rutin were determinate in P. edulis f. flavicarpa (1.61 mg/g extract and 0.58 mg/g dry plant), P. morifolia (2.10 mg/g extract and 0.90 mg/g dry plant), P. setacea (2.48 mg/g extract and 0.97 mg/g dry plant), P. setacea (8.46 mg/g extract and 3.30 mg/g dry plant) and P. galbana (3.48 mg/g extract and 1.02 mg/g dry plant), respectively. (C) 2017 Elsevier B.V. All tights reserved.
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