Journal
MICROBIOLOGY-SGM
Volume 163, Issue 11, Pages 1568-1577Publisher
MICROBIOLOGY SOC
DOI: 10.1099/mic.0.000539
Keywords
Candida albicans; Pseudomonas aeruginosa; bacteriophage; biofilm; iron
Categories
Funding
- National Science Foundation CAREER award [1453247]
- [3770]
- Bill & Melinda Gates Foundation - Grand Challenges Explorations Initiative [OPP1150703] Funding Source: researchfish
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1453247] Funding Source: National Science Foundation
- Bill and Melinda Gates Foundation [OPP1150703] Funding Source: Bill and Melinda Gates Foundation
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Pseudomonas aeruginosa (Pa) and Candida albicans (Ca) are major bacterial and fungal pathogens in immunocompromised hosts, and notably in the airways of cystic fibrosis patients. The bacteriophages of Pa physically alter biofilms, and were recently shown to inhibit the biofilms of Aspergillus fumigatus. To understand the range of this viral-fungal interaction, we studied Pa phages Pf4 and Pf1, and their interactions with Ca biofilm formation and preformed Ca biofilm. Both forms of Ca biofilm development, as well as planktonic Ca growth, were inhibited by either phage. The inhibition of biofilm was reversed by the addition of iron, suggesting that the mechanism of phage action on Ca involves denial of iron. Birefringence studies on added phage showed an ordered structure of binding to Ca. Electron microscopic observations indicated phage aggregation in the biofilm extracellular matrix. Bacteriophage-fungal interactions may be a general feature with several pathogens in the fungal kingdom.
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