Journal
SMALL METHODS
Volume 3, Issue 2, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smtd.201800474
Keywords
biomarkers; circulating tumor cells; magnetic force; near-infrared fluorescence; plasmonic chips
Funding
- National Natural Science Foundation of China (NSFC) [81771983, 81750110544, 81650110523]
- Shanghai Science and Technology Commission [16441909300]
- Ministry of Science and Technology of China [2017YFC0909000]
- Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning [TP2015015]
- Shenzhen Kongque program [KQTD20140630160825828, JSGG20160301095829250]
Ask authors/readers for more resources
Detection of circulating tumor cells (CTCs) in patient's blood is an important approach to cancer diagnosis and prognosis, but has been challenging due to the rarity of cells. Here, a magnetic-enhanced capturing of CTCs onto a plasmonic gold (pGOLD) chip, through a microfluidic immunomagnetic method, is demonstrated. Owing to the squashed/flattened morphology of cancer cells by magnetic forces and the resulting close proximity of near-infrared (NIR) labels on cells to the pGOLD surface, an ultrahigh NIR fluorescence enhancement of approximate to 50-120-fold is observed, drastically enhancing the ability of CTC detection, imaging, and analysis. Fluorescence enhanced, multiplexed protein biomarkers detection of CTCs is conducted for cancer cell spiked samples as well as CTCs in cancer patient's blood. Low CTC concentrations are detected down to approximate to 1 cell mL(-1) with capture efficiency up to approximate to 90%. Mechanical manipulation of cells by magnetic and other forces on plasmonic substrates represents a promising approach to ultrasensitive bio-analytical applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available