Enhanced production of target bioactive metabolites produced by Pseudomonas aeruginosa LV strain

Pseudomonas aeruginosa LV strain produces phenazine-1-carboxylic acid - PCA, phenazine-1-carboxamide - PCN, 2-carboxi-2-heptano-indol-3-ona - IDC, and fluopsin C - OAC. These molecules from secondary metabolism showed a potential for many biotechnological applications. The concentration of every compound obtained in the culture medium was below 6 mg L-1 and, in order to increase these concentrations a process of statistical optimization was carried out. Under optimum conditions the production of IDC, PCA, PCN and OAC increased 42, 38, 250 and 0.84 folds, respectively. The minimum inhibitory concentration of OAC against P. aeruginosa LV strain was 15 mg L-1, indicating that the concentration obtained (11.11 +/- 1.05 mg L-1) was very close to the maximum concentration possible in the medium. The optimized conditions presented here enhance the production of IDC, PCA, PCN and OAC by P. aeruginosa LV strain and should be implemented in this bacterial culture.