Journal
MICROBIAL PATHOGENESIS
Volume 104, Issue -, Pages 202-211Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2017.01.042
Keywords
Campylobacter jejuni; Cell shape
Categories
Funding
- Wellcome Trust [088786/C/09/Z, 098051]
- Isaac Newton Trust/Wellcome Trust ISSF/University of Cambridge
- Biotechnology and Biological Sciences Research Council [BB/K004514/1, BB/I02464X/1]
- Medical Research Council [MR/L015080/1]
- National Institute for Social Care and Health Research Fellowship [HF-14-13]
- Biotechnology and Biological Sciences Research Council [BB/I02464X/1, BB/K000055/1, BB/K004514/1] Funding Source: researchfish
- Medical Research Council [MR/L015080/1] Funding Source: researchfish
- BBSRC [BB/J017353/1, BB/I02464X/1, BB/K004514/1, BB/K000055/1, BB/I024674/1] Funding Source: UKRI
- MRC [MR/L015080/1] Funding Source: UKRI
Ask authors/readers for more resources
Campylobacter jejuni is the leading cause of bacterial food borne illness. While helical cell shape is considered important for C jejuni pathogenesis, this bacterium is capable of adopting other morphologies. To better understand how helical-shaped C jejuni maintain their shape and thus any associated colonisation, pathogenicity or other advantage, it is first important to identify the genes and proteins involved. So far, two peptidoglycan modifying enzymes Pgp1 and Pgp2 have been shown to be required for C. jejuni helical cell shape. We performed a visual screen of 2000 transposon mutants of C jejuni for cell shape mutants. Whole genome sequence data of the mutants with altered cell shape, directed mutants, wild type stocks and isolated helical and rod-shaped 'wild type' C jejuni, identified a number of different mutations in pgp1 and pgp2, which result in a change in helical to rod bacterial cell shape. We also identified an isolate with a loss of curvature. In this study, we have identified the genomic change in this isolate, and found that targeted deletion of the gene with the change resulted in bacteria with loss of curvature. Helical cell shape was restored by supplying the gene in trans. We examined the effect of loss of the gene on bacterial motility, adhesion and invasion of tissue culture cells and chicken colonisation, as well as the effect on the muropeptide profile of the peptidoglycan sacculus. Our work identifies another factor involved in helical cell shape. (C) 2017 The Authors. Published by Elsevier Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available