Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 7, Issue 8, Pages 1284-1291Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8tb03230k
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Funding
- National Natural Science Foundation of China [21405057, 21207047]
- Science and Technology Developing Foundation of Jilin Province of China [20180201050YY, 201701011106JC]
- Industrial Innovation Funds of Jilin Province of China [2018C034-1]
- Project of the Education Department of Jilin Province of China [2016411]
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Development of novel near-infrared (NIR) fluorescent probes for the monitoring of active substances in living organism is desirable in biological studies. Herein, we designed a novel NIR MTR fluorophore which has a longer emission wavelength, greater Stokes shift and higher quantum yield than the classic hemicyanine NIR fluorophore. The synthesized MTR-derived NIR probe (MTR-P) is highly selective and sensitive to alkaline phosphatase (ALP) activity. In the presence of ALP, MTR-P exhibited increased fluorescence signal by up to 56 fold at 723 nm, and it was determined to be 0.042 U L-1. In addition, the mechanism of the MTR-P probe was further examined by HPLC, mass spectrometry and DFT/TDDFT calculations. The NIR probe MTR-P was successfully applied to measure the levels of alkaline phosphatase in different types of cells by fluorescence imaging.
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