Journal
MICROBIAL CELL FACTORIES
Volume 16, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12934-017-0785-7
Keywords
Filamentous fungi; Protoplast-mediated transformation; Agrobacterium-mediated transformation; Electroporation; Biolistic method; Shock-wave-mediated transformation
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Funding
- National Natural Science Foundation of China [31301537, 31371287]
- Critical Patented Project of The Science & Technology Bureau of Fujian Province, China [2013YZ0002-1, 2013YZ0002-2]
- Science Development Foundation of Fuzhou University [2013-XY-17]
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Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking point for fungal genome modification. Up to date, there are some general methods of genetic transformation for fungi, including protoplast-mediated transformation, Agrobacterium-mediated transformation, electroporation, biolistic method and shock-wave-mediated transformation. This article reviews basic protocols and principles of these transformation methods, as well as their advantages and disadvantages.
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