Journal
METHODS
Volume 121, Issue -, Pages 77-85Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2017.03.005
Keywords
Knock-in; CRISPR/Cas9; Genome editing; Homologous recombination; Non-homologous-end-joining; Zebrafish
Funding
- ATIP/AVENIR program, ERC-StG [311159-Zebratectum]
- EU H Marie Sklodowska-Curie Action fellowship (H-MSCA-IF) [661527]
- Fondation Berthe Fouassier (Fondation de France) [2015-00060246]
- Marie Curie Actions (MSCA) [661527] Funding Source: Marie Curie Actions (MSCA)
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With its variety of applications, the CRISPR/Cas9 genome editing technology has been rapidly evolving in the last few years. In the zebrafish community, knock-out reports are constantly increasing but insertion studies have been so far more challenging. With this review, we aim at giving an overview of the homologous directed repair (HDR)-based knock-in generation in zebrafish. We address the critical points and limitations of the procedure such as cutting efficiency of the chosen single guide RNA, use of cas9 mRNA or Cas9 protein, homology arm size etc. but also ways to circumvent encountered issues with HDR insertions by the development of non-homologous dependent strategies. While imprecise, these homology-independent mechanisms based on non-homologous-end-joining (NHEJ) repair have been employed in zebrafish to generate reporter lines or to accurately edit an open reading frame by the use of intron-targeting modifications. Therefore, with higher efficiency and insertion rate, NHEJ-based knock-in seems to be a promising approach to target endogenous loci and to circumvent the limitations of HDR whenever it is possible and appropriate. In this perspective, we propose new strategies to generate cDNA edited or tagged insertions, which once established will constitute a new and versatile toolbox for CRISPR/Cas9-based knock-ins in zebrafish. (C) 2017 Elsevier Inc. All rights reserved.
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