Journal
METHODS
Volume 115, Issue -, Pages 55-64Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.11.016
Keywords
Image segmentation; Image co-localization; Fluorescence microscopy; Deconvolution; Spatial analysis; 3D imaging
Funding
- programme Investissements d'avenir [ANR-11-IDEX-0004-02]
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We present a new plugin for Image) called DiAna, for Distance Analysis, which comes with a user-friendly interface. DiAna proposes robust and accurate 3D segmentation for object extraction. The plugin performs automated object-based co-localization and distance analysis. DiAna offers an in-depth analysis of co-localization between objects and retrieves 3D measurements including co-localizing volumes and surfaces of contact. It also computes the distribution of distances between objects in 3D. With DiAna, we furthermore introduce an original method, which allows for estimating the statistical significance of object co-localization. DiAna offers a complete and intuitive 3D image analysis tool for biologists. (C) 2016 Elsevier Inc. All rights reserved.
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