Journal
METHODS
Volume 116, Issue -, Pages 23-33Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.11.008
Keywords
Recombinant antibody scFv; Panning; Purification
Funding
- Science Foundation Ireland under CSET [05/CE3/B754, 10/CE/B1821]
- Ireland under RECANT [CF/2015/0105]
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Recombinant antibodies are now very important in both therapeutics and diagnostics and offer significant advantages over conventional antibodies. The generation of a single-chain variable antibody fragment (scFv) (a common and important recombinant antibody format) is used to demonstrate the construction of a recombinant antibody library. An immunotube-based two-day panning approach, using Escherichia coli as an expression system, is utilised for antibody screening. The methods used for antibody selection and purification using immobilised metal affinity chromatography (IMAC) are described. (C) 2016 Elsevier Inc. All rights reserved.
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