Journal
METHODS
Volume 126, Issue -, Pages 76-85Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2017.05.027
Keywords
Microinjection; Fluorescent in situ hybridization; mRNA nuclear export kinetics; Mammalian tissue culture cells; Gene expression
Funding
- Canadian Institutes of Health Research [FRN 102725]
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2016-06607]
- Ontario Graduate Scholarship
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The nuclear export of mRNAs is an important yet little understood part of eukaryotic gene expression. One of the easiest methods for monitoring mRNA export in mammalian tissue culture cells is through the microinjection of DNA plasmids into the nucleus and monitoring the distribution of the transcribed product over time. Here we describe how to setup a microscope equipped with a micromanipulator used in cell microinjections, and we explain how to perform a nuclear mRNA export assay and obtain the nuclear export rate for any given mRNA. (C) 2017 Elsevier Inc. All rights reserved.
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