Journal
METHODS
Volume 120, Issue -, Pages 103-114Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2017.04.001
Keywords
Super-resolution imaging; Transcription; Quantitative microscopy; PALM; Single-particle tracking; Live cells
Funding
- UK EPSRC (through the University of Oxford LSI-DTC)
- BBSRC [BB/N018656/1]
- European Research Council [261227]
- UK BBSRC [BB/N018656/1, BB/J00054X/1]
- Wellcome Trust [110164/Z/15/Z]
- Wellcome Trust [204684/Z/16/Z] Funding Source: Wellcome Trust
- BBSRC [BB/J00054X/1, BB/H01795X/1, BB/N018656/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H01795X/1, BB/J00054X/1, BB/N018656/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1104939] Funding Source: researchfish
- Wellcome Trust [204684/Z/16/Z, 110164/Z/15/Z] Funding Source: researchfish
Ask authors/readers for more resources
In vivo single-molecule and super-resolution techniques are transforming our ability to study transcription as it takes place in its native environment in living cells. This review will detail the methods for imaging single molecules in cells, and the data-analysis tools which can be used to extract quantitative information on the spatial organization, mobility, and kinetics of the transcription machinery from these experiments. Furthermore, we will highlight studies which have applied these techniques to shed new light on bacterial transcription. (C) 2017 The Authors. Published by Elsevier Inc.
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