Journal
VETERINARY RESEARCH
Volume 50, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s13567-019-0632-4
Keywords
-
Categories
Funding
- European Reference Laboratory for Fish diseases at DTU AQUA National Institute of Aquatic Resources
- H2020 INFRAIA-1-2014/2015 Aquaexcel grant [652831]
- Norwegian Research Council (NRC) [237315/E40]
- Indian Council of Agricultural Research (ICAR) through the [National Bureau of Agriculturally Important Microorganisms (ICAR International Fellowship)]
- Norwegian Veterinary Institute (NVI)
Ask authors/readers for more resources
Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in different salmonid species. PRV-1 causes heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar) and is associated with jaundice syndrome in farmed chinook salmon (Oncorhynchus tshawytscha). PRV-2 causes erythrocytic inclusion body syndrome (EIBS) in coho salmon in Japan. PRV-3 has recently been associated with a disease in rainbow trout (Oncorhynchus mykiss) characterized by anaemia, heart and red muscle pathology; to jaundice syndrome in coho salmon (Oncorhynchus kisutch). In this study, we conducted a 10-week long experimental infection trial in rainbow trout with purified PRV-3 particles to assess the causal relationship between the virus and development of heart inflammation. The monitoring the PRV-3 load in heart and spleen by RT-qPCR shows a progressive increase of viral RNA to a peak, followed by clearance without a measurable change in haematocrit. The development of characteristic cardiac histopathological findings occurred in the late phase of the trial and was associated with increased expression of CD8+, indicating cytotoxic T cell proliferation. The findings indicate that, under these experimental conditions, PRV-3 infection in rainbow trout act similarly to PRV-1 infection in Atlantic salmon with regards to immunological responses and development of heart pathology, but not in the ability to establish a persistent infection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available