Journal
REPRODUCTION FERTILITY AND DEVELOPMENT
Volume 31, Issue 3, Pages 482-495Publisher
CSIRO PUBLISHING
DOI: 10.1071/RD18218
Keywords
extracellular matrix; proliferation; stroma
Categories
Funding
- Australian Government
- University of Adelaide
- National Health and Medical Research Council (NHMRC) of Australia
- NHMRC Centre for Research Excellence in the Evaluation, Management and Health Care Needs of Polycystic Ovary Syndrome
- Society for Reproductive Biology
- Robinson Research Institute
- Australian Research Council
- Griffith University
- S. Kidman and Co.
- Ridley Agriproducts Pty Ltd
- MRC [G1100357] Funding Source: UKRI
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During ovarian development stroma from the mesonephros penetrates and expands into the ovarian primordium and thus appears to be involved, at least physically, in the formation of ovigerous cords, follicles and surface epithelium. Cortical stromal development during gestation in bovine fetal ovaries (n=27) was characterised by immunohistochemistry and by mRNA analyses. Stroma was identified by immunostaining of stromal matrix collagen type I and proliferating cells were identified by Ki67 expression. The cortical and medullar volume expanded across gestation, with the rate of cortical expansion slowing over time. During gestation, the proportion of stroma in the cortex and total volume in the cortex significantly increased (P<0.05). The proliferation index and numerical density of proliferating cells in the stroma significantly decreased (P<0.05), whereas the numerical density of cells in the stroma did not change (P>0.05). The expression levels of 12 genes out of 18 examined, including osteoglycin (OGN) and lumican (LUM), were significantly increased later in development (P<0.05) and the expression of many genes was positively correlated with other genes and with gestational age. Thus, the rate of cortical stromal expansion peaked in early gestation due to cell proliferation, whilst late in development expression of extracellular matrix genes increased.
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