Journal
REPRODUCTION FERTILITY AND DEVELOPMENT
Volume 31, Issue 3, Pages 547-556Publisher
CSIRO PUBLISHING
DOI: 10.1071/RD18217
Keywords
caspase-3; Prochilodus lineatus; terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL)
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Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- CoordenacAo de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- FundacAo de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
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Fish embryos are particularly vulnerable to temperature changes, with the effects varying with developmental stage. The major aim of the present study was to analyse the relationship between apoptosis and heat shock protein (HSP) 70 during embryo development under thermal stress conditions. To this end, Prochilodus lineatus embryos at the blastopore closure stage were subjected to one of three thermal treatments for 1h (Group 1, 25 degrees C (control); Group 2, 20 degrees C; Group 3, 30 degrees C) and then examined at 0, 4 and 8h posttreatment (h.p.t.). The viability of embryos was highest in Group 1 (81.33 +/- 16.65%), followed by Group 3 and Group 2 (75.33 +/- 12.10% and 68.67 +/- 16.86% respectively), with significant difference between Groups 1 and 2 (P<0.05). At 0h.p.t., embryos subjected to thermal stress (Group 3) had a significantly higher number of terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL)- and caspase-3-labelled cells, and a lower number of HSP70-positive cells than those in the control group. At 4h.p.t., there was a decrease in the TUNEL reaction and an increase in HSP70 in embryos in Group 3. At 8h.p.t., the size of Group 3 embryos was significantly smaller than that of Group 1 embryos. The results indicate a cytoprotective role for HSP70, regulating caspase-3-mediated apoptosis during embryo development of P. lineatus; however, this mechanism is not effective in controlling embryo viability and larval malformations.
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