Journal
MEDICAL MOLECULAR MORPHOLOGY
Volume 51, Issue 2, Pages 111-117Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s00795-017-0178-3
Keywords
Dusp22; Dual-specificity phosphatase; Antibody; Mouse tissues
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Funding
- JSPS KAKENHI [16J06511, 23590124, 16K07211, 17K16294]
- Japan Agency for Medical Research and Development (AMED) [17bm0804009h0201]
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [17K16294, 16J06511, 16K07211, 23590124] Funding Source: KAKEN
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Dusp22 (dual-specificity phosphatase 22) is considered to regulate various cellular processes through the regulation of protein dephosphorylation. In this study, we prepared a specific antibody against Dusp22, anti-Dusp22, and carried out expression analyses with mouse tissues and cultured cell lines. Western blotting analyses demonstrated a tissue-dependent expression profile of Dusp22 in the adult mouse, and strongly suggested the presence of isoforms with larger molecular masses. In fibroblast NIH3T3 cells, while both endogenous and Myc-tagged Dusp22 was diffusely distributed in the cytoplasm, Myc-Dusp22 was partially colocalized with actin cytoskeleton. From the obtained results, anti-Dusp22 was found to be a useful tool for biochemical and cell biological analyses of Dusp22.
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