4.7 Article

Signs of biological activities of 28,000-year-old mammoth nuclei in mouse oocytes visualized by live-cell imaging

Journal

SCIENTIFIC REPORTS
Volume 9, Issue -, Pages -

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41598-019-40546-1

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Funding

  1. JSPS KAKENHI [JP25116005, JP18H05528, JP23500506, JP15H06753, JP16H01321, JP16H01222, JP17H05045, JP23580395, JP18H05527]
  2. MEXT-Supported Program for the Strategic Research Foundation at Private Universities, 2013-2017 [S1311017]
  3. Kindai University Grant KD15 [H23-25]
  4. Kindai University Grant KD10 [H26-28]

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The 28,000-year-old remains of a woolly mammoth, named 'Yuka', were found in Siberian permafrost. Here we recovered the less-damaged nucleus-like structures from the remains and visualised their dynamics in living mouse oocytes after nuclear transfer. Proteomic analyses demonstrated the presence of nuclear components in the remains. Nucleus-like structures found in the tissue homogenate were histone- and lamin-positive by immunostaining. In the reconstructed oocytes, the mammoth nuclei showed the spindle assembly, histone incorporation and partial nuclear formation; however, the full activation of nuclei for cleavage was not confirmed. DNA damage levels, which varied among the nuclei, were comparable to those of frozen-thawed mouse sperm and were reduced in some reconstructed oocytes. Our work provides a platform to evaluate the biological activities of nuclei in extinct animal species.

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