Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 17, Issue 11, Pages 2887-2891Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9ob00133f
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Funding
- Core Research for Evolutional Science and Technology (CREST) of Molecular Technology [JPMJCR13L4]
- Japan Science and Technology Agency
- KAKENHI [JP17K13265]
- Japan Society for the Promotion of Science
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Here, we report a facile permeability assay to quantitatively evaluate the membrane permeability of multiple peptides in parallel. With a fluorogenic click reaction between azidocoumarin and a terminal alkyne tag introduced on a peptide, the peptide that crossed an artificial membrane or a cell monolayer was quantitatively detected. The method allows a rapid measurement of the permeability of multiple compounds on a plate reader even in the presence of a complex mixture of biological molecules.
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