3.9 Article

DNA Extraction and Polymerase Chain Reaction

Journal

JOURNAL OF CYTOLOGY
Volume 36, Issue 2, Pages 116-117

Publisher

WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/JOC.JOC_110_18

Keywords

DNA extraction; Polymerase chain reaction; real time PCR

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DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient quantity. DNA can be amplified with the help of PCR. The basic principle and different variants of PCR are discussed.

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