Journal
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Volume 60, Issue 5, Pages 1769-1775Publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.18-25822
Keywords
health volunteers; aging; ocular surface; dry eye; para-inflammation
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Funding
- Italian Ministry of Health [RC263502]
- Regione Lazio (project MaBIOS-Kit) [FILAS-RU-2014-1112]
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PURPOSE. To assess the ocular surface in volunteers who consider themselves as healthy, in order to evaluate how para-inflammatory mechanisms fail with age, and thus investigate the phenomenon of InflammAging.'' METHODS. In this observational prospective cohort study, volunteers were categorized into three groups according to age: young (19-40 years), middle-aged (41-60 years), and older adults (61-93 years). Clinical assessments included tear breakup time (T-BUT) and Schirmer test type I. Dry eye symptoms were evaluated by the Ocular Surface Disease Index (OSDI) questionnaire. Conjunctival mRNA and protein expression of intercellular adhesion molecule-1 (ICAM-1), MUC5AC, and IL-8 were measured by real-time PCR and immunofluorescence. RESULTS. A total of 82 volunteers (38 males and 44 females) were enrolled. T-BUT decreased significantly with increasing age (young: 11.13 +/- 0.18 seconds; middle-aged: 10.83 +/- 0.56 seconds; older: 9.00 +/- 1.00 seconds, P < 0.05). Schirmer test values decreased significantly with age (young: 20.6 +/- 1.0 mm; middle-aged: 19.2 +/- 1.2 mm; older: 16.0 +/- 1.1 mm, P < 0.05). OSDI scores increased with age in both groups, but they were substantially higher in women. Conjunctival expression of inflammatory markers ICAM-1, IL-8, and MUC5AC increased with age. CONCLUSIONS. Clinical signs, symptoms, and biomarkers of chronic inflammation increased with age in a cohort of volunteers who considered themselves healthy, indicating an age-related progressive impairment of ocular surface system function.
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