3.9 Article

Evaluation of Antioxidant Capacities and Phenols Composition of Wild and Cultivated Berries

Journal

REVISTA DE CHIMIE
Volume 70, Issue 2, Pages 373-377

Publisher

CHIMINFORM DATA S A

Keywords

berries; total phenols; antioxidant capacity of soluble lipids; HPLC-DAD chromatography

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The study presents original results concerning analytical characterization of alcoholic extracts of four wild berries (blueberries, blackberries, red currants and raspberries) and two cultivated berries (black cherries and strawberries). Total and some individual phenols concentrations were determined using molecular absorption spectrometry (modified Folin Ciocalteu method) and HPLC-DAD technique. The measurement of lipid-soluble antioxidant capacity (ACL) was done by photochemiluminescence method using trolox as standard. The highest total phenolic content was registered in blueberries (543.5 mg/100g f.w.), while the lowest was found in raspberries (344.5 mg/100g f.w.).HPLC - DAD analysis indicated the presence of six individual polyphenolic compounds in different concentrations: gallic add (62.664 - 178.821 mg/100 g f.w); chlorogenic acid (30.152 - 243.923 mg/100g f.w.); 3-O-methyl-gallic acid (2.035 - 4.907 mg/100 g f.w.); caffeic add (0.401 - 5.664 mg/100 g f.w.); p-coumaric add (4.252 - 10.806 mg/100 g f.w.) and cinnamic add (0.665-0.958 mg/100 g f.w.). ACL results showed values between 54.00 and 1152 mu mols trolox/100 g f.w.

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