Journal
ANALYST
Volume 144, Issue 6, Pages 1955-1959Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8an02368a
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [31671923, 81873539]
- Shanghai Pujiang Program [18PJD016]
Ask authors/readers for more resources
As a bifunctional enzyme, T4 polynucleotide kinase phosphatase (T4 PNKP) catalyzes the phosphorylation of 5'-hydroxyl, and also removes the terminal 3'-phosphate group. This is closely related to the restructuring, replication, and damage repair of nucleic acid. In this paper, we describe a new method for the sensitive detection of T4 PNKP activity based on the isothermal EXPonential amplification reaction (EXPAR). T4 PNKP can be linearly assayed in the range from 0.001 to 0.01 U mL(-1) with a detection limit of 7.9 x 10(-4) U mL(-1). Moreover, the method exhibits high specificity and sensitivity and can be applied in the enzyme analysis of complex serum samples. In view of its simplicity and moderate experimental conditions, the method may suitable for use in a commercial kit for the analysis of T4 PNKP activity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available