Journal
CHEMICAL COMMUNICATIONS
Volume 55, Issue 24, Pages 3540-3543Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8cc10132a
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Funding
- Natural Sciences and Engineering Research Council of Canada [2018-04621]
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The coumarin-hemicyanine hybrid is an internal charge transfer (ICT) dye that features a donor--acceptor motif and displays two well-resolved absorption bands at approximate to 350 nm for the donor coumarin and approximate to 550 nm for the fully-conjugated dye. Herein, we demonstrate that preferential excitation of the dye at 350 nm, ascribed to a twisted conformation, gives rise to two well-resolved emission bands at approximate to 450 nm for the coumarin and approximate to 650 nm for the planar dye that are highly quenched in water. Unexpectedly, the coumarin blue emission was found to turn-on' to aprotic and viscous solvents and respond to binding bovine serum albumin (BSA) in aqueous media. In contrast, the red ICT emission was sensitive to protic solvent polarity and responded to binding the G-quadruplex (GQ) DNA structure produced by the heme binding aptamer (PS2.M). Our studies also demonstrate the influence of the alkyl group attached to the dye quaternary nitrogen atom in determining the microenvironment sensitivity and ability to differentiate biopolymer types.
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