Carbon and Nitrogen Sources for Lipase Production of Micrococcus sp. Isolated from Palm Oil Mill Effluent-Contaminated Soil

This research aim to optimize the lipase production of Micrococcus sp. isolated from oil palm contaminated soil. The production of lipase enzymes is carried out in modified medium containing olive oil, sea salt, yeast extract with various types of carbon source (glucose, sucrose, glycerol) and nitrogen sources (tryptone, ammonium phosphate, urea and ammonium nitrate). The activity of lipase enzyme was determined by UV-Vis spectrophotometric method toward rho-nitrophenylpalmitate (rho-NPP) as a substrate. The results showed that glucose 1% was better carbon source compared with sucrose and glycerol. Ammonium phosphate, urea and ammonium nitrate were good nitrogen sources for lipase production of Micrococcus sp., although tryptone was the best nitrogen source.