Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 75, Issue -, Pages 629-636Publisher
ELSEVIER
DOI: 10.1016/j.msec.2017.02.091
Keywords
Immobilization; Magneto-elastic; Biosensor; Resonant frequency; Atomic force microscopy; Self-assembled monolayer
Categories
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [447777/2014-9]
- CNPq [306073/2014-5]
- CAPES
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Magneto-elastic biosensors have mass sensitivity to biological species, offering reliability and reproducibility in the detection of pathogens such as Escherichia coli. In this work, amorphous ribbons of Metglas 2826MB3 were coated with layers of Cr and Au by DC magnetron sputtering and cut to 5 mm x 1 mm. The influence of different thiols on captured pathogens was studied. The compounds cystamine (CYS), cysteamine (CYSTE) and mercaptopropionic acid (MPA) were deposited on Au-covered surfaces, followed by antibodies. The roughness parameters Ra and Rq were determined using atomic force microscopy (AFM) and micrographs from scanning electron microscopy with a field emission gun (FESEM) were also utilized. Biosensors formed with MPA showed an increased efficiency for attracting E. coli compared to biosensors with CYS and CYSTE, but large standard deviations were observed, making reproducibility and reliability difficult for that biosensor. Sensors tested with CYSTE showed greater efficiency and a lower detection limit than sensors with CYS. The results indicated that the size of the carbon chain and the terminal grouping influence the effectiveness of immobilization on magneto-elastic biosensors. (C) 2017 Elsevier B.V. All rights reserved.
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