Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 75, Issue -, Pages 610-619Publisher
ELSEVIER
DOI: 10.1016/j.msec.2017.02.080
Keywords
AgO; Bactericidal mechanism; Membrane damage; Morphological changes; Antimicrobial properties
Categories
Funding
- Xi'an University of Technology [101-256211414]
- Key Project of Agricultural Science and Technology Innovation of Shaanxi Province [2015NY060]
- Key Laboratory Project of Education Department of Shaanxi Province [15JS080]
- Science and Technology Co-ordination & Innovation Key Laboratory Project of Shaanxi Province [2014SZS09-Z02]
- Program of Shaanxi Academy of Sciences [2016K-24]
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To identify the mechanistic effects of AgO nanoparticles on Gram-positive bacteria, S. aureus cells suspended in phosphate buffer solution (PBS) and deionized water were separately treated using AgO nanoparticles at different concentrations. The phase composition changes of the bactericide after killing S. aureus and the cellular responses of S. aureus to AgO were characterized by X-ray diffraction, atomic absorption spectrophotometer, scanning electron microscopy, transmission electron microscopy, and energy dispersive spectroscopy. The results show that AgO nanoparticles could kill S. aureus suspended in PBS and deionized water. The bactericidal effect of AgO bactericide against S. aureus in water was better than that in PBS, due to the formation of Ag3PO4 from the reaction between AgO and PBS. AgO nanoparticles exerted their bactericidal activity by multiple processes. AgO nanoparticles adhered to the surface of S. aureus cells firstly, then induced physical alterations in cell morphology and released silver ions, leading to initial injuries of cell membrane. Once membrane damage occurred, they entered the cells, and damaged the intracellular materials, eventually causing severe morphological and structural injuries to the cells and leakage of cytoplasm. (C) 2017 Elsevier B.V. All rights reserved.
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