Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 79, Issue -, Pages 93-99Publisher
ELSEVIER
DOI: 10.1016/j.msec.2017.05.012
Keywords
Epinephrine; Carbon nanotubes; Chitosan; Raman spectroscopy; Electrochemical biosensor; Artificial urine
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Funding
- National Research Foundation of Korea (NRF) - Ministry of Education, Republic of Korea [2009-0093816]
- Ministry of Human Resource Development India
- National Institute of Technology Warangal, India
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An efficient electrochemical sensor for selective detection of the neurotransmitter, epinephrine (Epn), has been fabricated with the aid of a functionalized multiwall carbon nanotube-chitosan biopolymer nanocomposite (Chit-fCNT) electrode. Multiwall carbon nanotubes (CNT) were successfully functionalized with the aid of nitric acid and confirmed by the Raman spectral data. Functionalized carbon nanotubes (fCNT) were dispersed in chitosan solution and the resulting bio-nanocomposite was used for the fabrication of sensor surface by drop and cast method. Electrochemical characteristics of the fabricated sensor were understood using cyclic, differential pulse voltammetry (CV, DPV) and electrochemical impedance analysis for the detection of Epn in phosphate buffer (pH 7.4). CV and impedance analysis revealed that the Chit-fCNT modified electrode enhances the electrodic reaction of Epn and facilitated the electron transfer more readily compared to that of bare electrode. Applying DPV for the detection of Epn, achieved 30 nM as the lowest detection limit in the determination range of 0.0510 M and the analytical time as low as 10 s. Selective determination of Epn against the coexistence of a number of biological electroactive interferents and reproducible results for the determination of Epn were demonstrated. The present biosensor has been found efficient for successful direct determination of Epn from pharmaceutical adrenaline formulations and urine samples. (C) 2017 Elsevier B.V. All rights reserved.
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