A novel nano-hydroxyapatite - PMMA hybrid scaffolds adopted by conjugated thermal induced phase separation (TIPS) and wet-chemical approach: Analysis of its mechanical and biological properties

In this study, we report the preparation of nano-hydroxyapatite (nHAp) incorporated poly(methyl methacrylate) (PMMA) scaffolds by conjugated thermal induced phase separation (TIPS) and wet -chemical approach, which essentially facilitates the enhancement of both mechanical as well as biological properties of the scaffolds. The dissolution of PMMA was accomplished by acetone (Ace scaffold), ethanol-water (E-W scaffold) and isopropanolwater (I-W scaffold) mixtures as solvents. The existence of nHAp in PMMA matrix was investigated systematically. The porosity of similar to 57.89% was achieved from Ace scaffold that was higher degree compared to both I-W and E-W scaffolds. On the other hand, the dense porous architecture of I-W scaffold exhibited superior hardness of similar to 65.6 HR 'D' than that of the Ace and E-W scaffolds. All the fabricated samples demonstrated enhanced in vitro bioactivity with respect to increasing immersion period as a result of flower-like in vitro apatite layer formation. The MTT assay was carried out for 1 day and 3 day culture using Saos-2 osteoblast-like cells, which showed better cell proliferation with increasing culture period owing to the interconnected pore architecture of scaffolds and the rational hemocompatibility as per the ASTM standard F756-00. (C) 2016 Elsevier B.V. All rights reserved.