Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 70, Issue -, Pages 505-511Publisher
ELSEVIER
DOI: 10.1016/j.msec.2016.09.020
Keywords
PLGA; Hyperspectral fluorescence; Two-photon microscopy; Biodistribution; Cresyl violet
Categories
Funding
- CNPq [246919/2012-4, 246920/2012-2, 4710 78/2012-3]
- CAPES [23106.006580/2012-16]
- FAPDF [193.000890/2015]
- FINATEC
- Fundacao Lemann
- IANAS
- NSF [0954796]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0954796] Funding Source: National Science Foundation
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Here we present the production, characterization and in-vivo assessment of cresyl violet-loaded biodegradable PLGA nano/microparticles (CV-NP and CV-MP). We demonstrate that the beneficial spectral characteristics of cresyl violet make it suitable as a tracer for particle-based drug delivery using both hyperspectral wide field and two-photon excited fluorescence microscopy. Particles were prepared using a cosolvent method, after which the physicochemical properties such as morphology, particle size, drug entrapment efficiency, drug loading and in vitro drug release behavior were measured in addition to spectroscopic properties, such as absorption, fluorescence and infrared spectra. The particles were then tested in an in vivo mouse model to assess their biodistribution characteristics. The location and integrity of particles after injection was determined using both hyperspectral fluorescence and two-photon microscopy within intact organs in situ. Our results show that cresyl violet is efficiently entrapped into PLGA particles, and that the particles are spherical in shape, ranging from 300 to 5070 nm in diameter. Particle biodistribution in the mouse was found to depend on particle size, as expected. Cresyl violet is shown to be an ideal tracer to assess the properties PLGA particle-based drug delivery in combination with our novel multi-scale optical imaging techniques for in-situ particle localization. (C) 2016 Elsevier B.V. All rights reserved.
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