Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 77, Issue -, Pages 184-189Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2017.03.222
Keywords
Hydrogel; Bladder acellular matrix; Growth factors
Categories
Funding
- National Natural Science Foundation of China [21674018, 81671451, 81600524]
- Shuguang Program - Shanghai Education Development Foundation
- Shanghai Municipal Education Commission [15SG30]
- DHU Distinguished Young Professor Program [A201302]
- Fundamental Research Funds for the Central Universities
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University [LK1408]
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Bladder acellular matrix (BAM) hydrogel may have great potential in tissue engineering due to outstanding biocompatibility and the presence of inherent bioactive factors in BAM. In this study, we prepared the BAM hydrogel by the method of enzymatic solubilization with pepsin and characterize the microrheological properties of the BAM precursor solution. The structures of the BAM hydrogel were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). Furthermore, the growth factors including vascular endothelial growth factor (VEGF), platelet-derived growth factor B (PDGF-BB), keratinocyte growth factor (KGF) were quantified by ELISA. The biological performances of the hydrogels were evaluated by cultivating porcine iliac endothelial cells (PIECs) in vitro. Lyophilized BAM showed porous structure with pore diameter ranging from 50 to 100 pm. BAM 4-G hydrogel (4 mg/mL) with a short gelation time of 3.95 +/- 0.07 min presents better thermal stability than BAM 6-G hydrogel (6 mg/mL). Growth factors in the BAM hydrogel maintain valuable biological activity even after digestion process. The BAM hydrogel supported the adhesion and growth of PIECs well and has great potential for further tissue engineering. (C) 2017 Elsevier B.V. All rights reserved.
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