Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 71, Issue -, Pages 862-869Publisher
ELSEVIER
DOI: 10.1016/j.msec.2016.10.078
Keywords
Poly(lactic) acid; Poly(acrylic) acid; Surface modification; Entrapment; Composite materials; Fluorescent compounds; Bovine serum albumin
Categories
Funding
- Russian Science Foundation [16-13-10239]
- PLA-PAA [4.1991.2014/K]
- RFBR [16-33-00528]
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A new approach for the immobilization of poly(acrylic) acid (PAA) as a chemically reactive cross-linker on the surface of poly(lactic) acid-based (PLA) biomaterials is described. The proposed technique includes non covalent attachment of a PAA layer to the surface of PLA-based biomaterial via biomaterial surface treatment with solvent/non-solvent mixture followed by the entrapment of PAA from its solution. Surface morphology and wettability of the obtained PLA-PAA composite materials were investigated by AFM and the sitting drop method respectively. The amount of the carboxyl groups on the composites surface was determined by using the fluorescent compounds (2-(5-aminobenzo[d]oxazol-2-yl)phenol (ABO) and its acyl derivative N-(2-(2-hydroxyphenyl)benzo[d]oxazol-5-yl)acetamide (AcABO)). It was shown that it is possible to obtain PLA-PAA composites with various surface relief and tunable wettability (57 degrees, 62 degrees and 66 degrees). The capacity of the created PM layer could be varied from 1.5 nmol/cm2 to 0.1 mu mol/cm(2) depending on the modification conditions. Additionally, using bovine serum albumin (BSA) it was demonstrated that such composites could be modified with proteins with high binding density (around 0.18 nmol/cm(2)). Obtained fluoro-labeled PLA-PAA materials, as well as PLA-PM composites themselves, are valuable since they can be used for biodegradable polymer implants tracking in living systems and as drug delivery systems. (C) 2016 Elsevier B.V. All rights reserved.
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