Journal
ACS APPLIED BIO MATERIALS
Volume 2, Issue 4, Pages 1591-1602Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.9b00020
Keywords
poly(vinylidene fluoride-co-trifluoroethylene); topography; morphology; cell adhesion; myoblasts; preosteoblasts
Funding
- Portuguese Foundation for Science and Technology (FCT) [UID/FIS/04650/2013, UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569), POCI-01-0145-FEDER-028237, POCI-01-0145-FEDER-028159]
- ERDF through the COMPETE2020 - Programa Operacional Competitividade e Internacionalizacao (POCI)
- European Regional Development Fund under the Norte2020 - Programa Operacional Regional do Norte [NORTE-01-0145-FEDER-000004, UID/BIO/04469, POCI-01-0145-FEDER-006684]
- FCT [SFRH/BPD/98109/2013, SFRH/BD/111478/2015, SFRH/BPD/90870/2012]
- Spanish Ministry of Economy and Competitiveness (MINECO) [MAT-201676039-C4-3-R]
- Basque Government Industry and Education Department under the ELKARTEK program
- Basque Government Industry and Education Department under the HAZITEK program
- Basque Government Industry and Education Department under the PIBA program
- Fundacao para a Ciencia e a Tecnologia (FCT)
- Fundação para a Ciência e a Tecnologia [SFRH/BD/111478/2015] Funding Source: FCT
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Electroactive polymers are being increasingly used in tissue engineering applications. Together with the electromechanical clues, morphological ones have been demonstrated to determine cell proliferation and differentiation. This work reports on the micropatterning of poly(vinylidene fluoride-co-trifluoroethylene), P(VDF-TrFE) scaffolds, and their interaction with myoblast and preosteoblasts cell lines, selected based on their different functional morphology. The scaffolds were obtained by soft lithography and obtained in the form of arrays of lines, intermittent lines, hexagons, linear zigzags, and curved zigzags with dimensions of 25, 75, and 150 mu m. Moreover, the scaffolds were tested in cell adhesion assays of myoblasts and preosteoblasts cell lines. The results show that more linear surface topographies and dense morphology have a large potential in the regeneration of musculoskeletal tissue, while nonpatterned scaffolds or more anisotropic surface microstructures present largest potential to promote the growth and regeneration of bone tissue. In this way, cell adhesion site, orientation, and elongation can be controlled by choosing properly the topography and morphology of the scaffolds, indicating their suitability and potential for further proliferation and differentiation assays.
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