Journal
ACS APPLIED BIO MATERIALS
Volume 2, Issue 4, Pages 1406-1412Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.8b00686
Keywords
xeno-free nanofibrous scaffolds; ultrashort peptides; self-assembled peptide hydrogels; human pluripotent stem cells; directed 3D differentiation
Funding
- Institute of Bioengineering and Nanotechnology
- Genome Institute of Singapore
- Biomedical Research Council (Agency for Science, Technology and Research, Singapore)
- Agency for Science, Technology and Research (A*STAR) Joint Council Office (JCO) [1231AFG023]
- A*STAR BMRC YIG grant [14/1/07/51/011]
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Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation, and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol-gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.
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