Journal
ISCIENCE
Volume 13, Issue -, Pages 305-+Publisher
CELL PRESS
DOI: 10.1016/j.isci.2019.02.028
Keywords
-
Categories
Funding
- Robert Bosch Innovation Centre Grant [RBCO0014]
- DBT-IISC Partnership Program [BT/PR27952/INF/22/212/2018]
- Council for Scientific and Industrial Research CSIR [CSIR-JRF 09/079(2764)/2017-EMR-1]
Ask authors/readers for more resources
Swarming in Pseudomonas aeruginosa is a coordinated movement of bacteria over semisolid surfaces (0.5%-0.7% agar). On soft agar, P. aeruginosa exhibits a dendritic swarm pattern, with multiple levels of branching. However, the swarm patterns typically vary depending upon the experimental design. In the present study, we show that the pattern characteristics of P. aeruginosa arm are highly environment dependent. We define several quantifiable, macroscale features of the arm to study the plasticity of the swarm, observed across different nutrient formulations. Furthermore, through a targeted screen of 113 two-component system (TCS) loci of the P. aeruginosa strain PA14, we show that forty-four TCS genes regulate swarming in PA14 in a contextual fashion. However, only four TCS genes-fleR, fleS, gacS, and PA14_59770-were found essential for swarming. Notably, many swarming-defective TCS mutants were found highly efficient in biofilm formation, indicating opposing roles for many TCS loci.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available