4.7 Article

Effect of carbon sources for PHB production in bubble column bioreactor: Emphasis on improvement of methane uptake

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ELSEVIER SCI LTD
DOI: 10.1016/j.jece.2019.102978

Keywords

PHB production; Gaseous substrates; Loop bioreactor; Methane; Mixed carbon sources; Natural gas

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In this study, poly(3-hydroxybutyrate) (PHB) production was investigated for the first time using high cell density cultivation of Methylocystis hirsuta from natural gas. Various carbon sources including acetate, ethanol and glucose were used in a loop bioreactor. Then, effects of magnesium and phosphorus contents on PHB production were investigated. Results revealed that the bacterial strain had the capability to grow on various substrates. Furthermore, the cell dry mass (CDM) reached to 4.5 g/L with a PHB content of 85% (w/w) using a ratio of 1: 1 of methanol: ethanol. Deficiencies of magnesium and phosphorus were shown to play a key role to achieve high cell densities in bioreactors. In addition, PHB was produced in a bubble column bioreactor at optimum conditions, resulting in the production of 8 g/L of CDM with a PHB content of 73.4% (w/w).

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