Journal
NEW JOURNAL OF CHEMISTRY
Volume 43, Issue 14, Pages 5297-5307Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8nj05418e
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Funding
- DST, West Bengal [809(Sanc)/ST/P/ST4G9/2104]
- CSIR, New Delhi, India [01(2896)/17/EMR-II]
- UGC-MANF [2012-13-MANF-MUS-WES-13502]
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A fluorescein hydrazone based conjugate (H2L3) was synthesized by coupling fluorescein hydrazide (L-1) with 2-(pyridin-2-ylmethoxy)-naphthalene-1-carbaldehyde (L-2). H2L3 was well characterized by several spectroscopic methods such as IR, H-1, C-13-NMR and ESI-MS. The probe H2L3 exhibited high selectivity and high sensitivity towards toxic Hg2+ ions in semi-aqueous medium (pH 7.2, 10 mM HEPES) over other metal ions. The significant enhancement in fluorescence emission centered at 520 nm was attributed to the Hg2+-induced ring opening of the spirolactam moiety in the fluorescein structure. The 1:1 binding of H2L3 to Hg2+ was established by Job's method and confirmed by ESI-MS+ (m/z) studies and the binding constant was calculated as (0.43 +/- 0.04) x 10(4) M-1 with a detection limit of 1.24 M. Then again, the [Hg(HL3)](+) ensemble could be utilized as a reversible fluorescent sensor for S2-. On addition of S2- to the [Hg(HL3)](+) complex, the fluorescence intensity was totally quenched because Hg2+ in the complex was grabbed by S2- because of the stronger binding force between Hg2+ and S2-. The tentative coordination environment in the [Hg(HL3)](+) complex was established by DFT studies. The fluorescence OFF-ON-OFF mode of H2L3 was examined in the presence of Hg2+ and S2- and finds applications in devices with logic gate functions. H2L3 also exhibits bio-compatibility and negligible cytotoxicity and is suitable for fluorescence cell imaging of Hg2+ ions in live HepG2 cells.
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